V. Vállez-Gomis, G. Peris-Pastor, J.L. Benedé, A. Chisvert, A. Salvador
B vitamins are a group of compounds with beneficious properties for dermatologic care, and therefore they are included in the cosmetic formulations as high added-value ingredients. In this paper, an analytical method for the simultaneous determination of eight water-soluble B vitamins in cosmetic products is reported for the first time. This method is based on liquid chromatography with ultraviolet detection (LC-UV) analysis after simple water leaching of the analytes from the cosmetic matrix. No organic solvents are required, beyond the ethanol used in the chromatographic mobile phase. The proposed method has been successfully validated showing good linearity, limits of detection in the low μg mL−1 range (from 0.14 to 0.43 μg mL−1) and good repeatability (relative standard deviation below 11 %). The accuracy of the method has been proven by the analysis of laboratory-made samples (i.e., a cream and a gel) with known concentrations of the analytes, providing low relative errors (below 12 %). Finally, the method has been successfully applied to four commercial cosmetic samples of different formulations without significant matrix effects. The results obtained and the environmentally friendly features of the method showed its usefulness for carrying out both pre- and in-market quality control of final cosmetic products.
Keywords: B vitamins; Cosmetic products; Liquid chromatography; Water-soluble vitamins.
Green determination of eight water-soluble B vitamins in cosmetic products by liquid chromatography with ultraviolet detection. V. Vállez-Gomis, G. Peris-Pastor, J.L. Benedé, A. Chisvert, A. Salvador, J. Pharm. Biomed. Anal. 205 (2021) 114308.